Mapping of the genes around MEN2A locus using pulsed-field gel electrophoresis
نویسندگان
چکیده
منابع مشابه
Pulsed-field gel electrophoresis.
DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
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The technique of pulsed-field gradient electrophoresis (PFGE) allows the determination of gene linkage relationships since DNA fragments up to 2 Mb can be separated. PFGE was employed to study linkage of class I, II and III genes belonging to the human major histocompatibility (HLA) complex. The results establish that the class II DO beta and DZ alpha genes are linked with the DP subregion, cen...
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Pulsed field gel electrophoresis (PFGE) is capable of resolving large DNA molecules of up to two million nucleotides and is becoming a widely used technique in DNA studies (1—3). Three factors can potentially limit PFGE in a laboratory: 1. the cost of the apparatus, 2. reproducibility of electrophoretic conditions, and 3. unusually long electrophoretic time. To alleviate some of these problems ...
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Mobility of supercoiled (form I) and nicked circular (form II) plasmid DNAs was determined on two major forms of pulsed-field electrophoresis, CHEF and OFAGE. Plasmids with molecular lengths ranging from 2.30 to 17.8 kilobase pairs (kb) were used with Saccharomyces cerevisiae chromosomes as standards. Agarose gel concentrations were varied from 0.3 to 2.0 percent, with higher percentage gels re...
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ژورنال
عنوان ژورنال: Japanese journal of human genetics
سال: 1990
ISSN: 0021-5074
DOI: 10.1007/bf01883755